Purification and Characterization of a Glutathione S-Transferase from Pome Fruits
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چکیده
منابع مشابه
Purification and characterization of a new cytosolic glutathione S-transferase (glutathione S-transferase X) from rat liver.
A hitherto unknown cytosolic glutathione S-transferase from rat liver was discovered and a method developed for its purification to apparent homogeneity. This enzyme had several properties that distinguished it from other glutathione S-transferases, and it was named glutathione S-transferase X. The purification procedure involved DEAE-cellulose chromatography, (NH4)2SO4 precipitation, affinity ...
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An intracellular glutathione transferase was purified to homogenity from the fungus, Mucor mucedo, using DEAE-cellulose ion-exchange and glutathione affinity chromatography. Gel filtration chromatography and SDS-PAGE revealed that the purified GST is a homodimer with approximate native and subunit molecular mass of 53 kDa and 23.4 kDa, respectively. The enzyme has a pI value of 4.8, a pH optimu...
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ژورنال
عنوان ژورنال: HortScience
سال: 2004
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.39.4.781c